The system has loaded the relevant variables of relion by default, enter the command in the terminal: relion can open the GUI interface, and then follow the steps to use normally (must be in the login account can read and write directory).
Relion tutorial：ftp://ftp.mrc-lmb.cam.ac.uk/pub/scheres/relion30_tutorial.pdf
For our GPU workstation, it was suggested to set the computing parameter like this:
Preprocessing (GPU acceleration job): MPI=[graphic card number]
refine (new 2080Ti): MPI=2*[graphic card number]+1
refine (old 1080Ti): MPI=[graphic card number]+1
threads: 3~5 would be fine for refine(2D 3D job), 6~9 would be fine for preprocessing.
pooled particles: 10~100 when using GPU acceleration, 2*threads when using CPU acceleration
Copy particles to scratch directory: /ssd
Here are the instruction pictures (using mainstream workstation for example):
Motion Correction running tab:
Refine compute tab:
In the running tab, when running relion_refine:
Tips：
When stopping or deleting task in GUI, the relion process will still exist in the background (it will take up resources), need to manually stop/delete the related process to release the resource;
Command "ps -ef | grep relion" can query all the relion processes and find the PID for the just stop or del relion task. Use the command "Kill PID" to delete the corresponding process.
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